Using an Experimental Technique Essay Example for Free

exploitation an Experimental Technique EssayA nonher chemical commonly used is ethanol in the unfertile technique and this too was varied in the amount of time the seeds were soaked earlier being dictated in varying times and concentrations of the sodium hypochlorite. Ultimately, in order to use the barleycorn seeds we need seduce a sterilization technique that can eliminate majority of fungal contamination while not harming the germination of the chosen seeds. I propose an experimental idea to eliminate fungal contamination from barley seed germination. Materials and Methods Aseptic Technique The working area was sprayed down completely with 95% Ethanol before the seeds were used. The forceps used to transfer the seeds from liquid to media were sterilized using the ethanol. Seed Sterilization Procedure Using aseptic techniques 20 barley seeds obtained from the paper bag kept in the fridge were transferred into sterile distilled water and let to sit in a drawer for 20 ho urs overnight before being transferred to 100% decolourise with a drop of dish liquid (Inatomi and Slaughter, 1971). The seeds were soaked in the bleach for a total of 2 hours before being dipped in sterile distilled water and fixed on Petri dishes with minimal media and placed in a growth chamber.The germination of the seeds was monitored for 7 days for any fungal contamination. Results After 3 days the seeds were germinating (13 out of 20) and thither was no visible evidence of fungal growth. 5 age there were no contaminants. 7 Days no contaminants. 3 Days seed growth. No contaminants. 3 Days seed growth. No contaminants. 5 Days seed growth. No contaminants. 5 Days seed growth. No contaminants. Discussion Fungal contaminations can be a problem when trying to germinate seeds and a victorious experiment is needed to sterilize the barley seeds for further experimentation.I proposed a sterilization experiment for barley seeds and had success with 65% germination and 0% fungal growth. This sterilization technique could provide a practiced beginning point to begin other experimentation on germination success of barley seeds, without the provoke of fungal contamination to destroy possible results. The overnight soak in the water forced the fungal spores to begin germinating and thereby making it possible for the 2 hour bleach soak to become to a greater extent effective in eliminating the fungus and other contaminants from the seed coat.The few seeds which did not germinate could have had too a good deal bleach penetration and therefore killed these seeds, however the success was more beneficial than the failure, and so this novel sterilization technique was effective in its elimination of fungal contaminations while providing a good germination place of the barley seeds. Literature Cited K. Inatomi and J. C. Slaughter. 1971. The Role of Glutamate Decarboxylase and Aminobutyric Acid in Germinating Barley. J. Exp. Bot. 22561-571.