Chromatography of Dyes :: Chromatography Experiments Dye Essays

Chromatography of DyesAn extremely common technique by using 2 chemicals and filter publisher can wear out you sufficient information. A well-done Chromatography of discolors willing provide you with enough info to determine if a liquid mixture is heterogeneous or same . In a learning activity you can separate and maybe identify food dyes in some boozings mixes and food colors. The effect of clo certain polarity or the ability to separate and elute compounds will alike be demonstrated. In this experiment you will need to use the comparison Xcm/Acm=RF value. The length of A, is the length of the longest line of the food dye or food coloring. The Length of X is the length of all the another(prenominal) vari opens. In the experiment the lengths will be mea reliabled in Centimeters. experimental ProcedureYou will need to obtain a piece of received filter paper approximately 10*15 cm. Use a pencil to draw a line parallel to one of the long-run edges, about 1 cm from the ed ge. Make 10 dashes on the pencil line, be sealed to have them evenly distributed. At this point you will spot each of the 5 standards on separate dashes. Be sure to write down which liquid is at which dash. Then choose two food colors and two drink mixes, and spot them on the be four dashes. Curl the filter paper into a cylinder, make sure colors out, and staple the edges together. Do not allow the edges of the filter paper to overlap. Add approximately 10 ml of a 73 isopropanolwater mixture to your 600 mL beaker, then carefully come to the fore the chromatogram into the beaker the colored spots must be higher than the upshot. Be sure to cover the beaker, remove, lay out to dry then measure when the solvent is 1 to 2 cm from the top. Repeat all the above steps but use 0.1% NaCl in the place of the 73 isopropanol. By comparing the colors of the spots in the standards and the unknowns, and the distances the spots moved, you should be able to tell what compounds are present in the food colors and drink mixes. Some of the food colors and drink mixes may contain merely one dye, and some may contain more than one.